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Structural response of the cornea to changes in stromal hydration

Hayes, Sally, White, Tomas, Boote, Craig, Kamma-Lorger, Christina S., Bell, James Stephen, Sorenson, Thomas, Terrill, Nick, Shebanova, Olga and Meek, Keith Michael Andrew 2017. Structural response of the cornea to changes in stromal hydration. Journal of the Royal Society Interface 14 (131) 10.1098/rsif.2017.0062

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Abstract

The primary aim of this study was to quantify the relationship between corneal structure and hydration in humans and pigs. X-ray scattering data were collected from human and porcine corneas equilibrated with polyethylene glycol (PEG) to varying levels of hydration, to obtain measurements of collagen fibril diameter, interfibrillar spacing and intermolecular spacing. Both species showed a strong positive linear correlation between hydration and interfibrillar spacing2 and a non-linear, bi-phasic relationship between hydration and fibril diameter, whereby fibril diameter increased up to approximately physiological hydration, H = 3.0, with little change thereafter. Above H = 3.0, porcine corneas exhibited a larger fibril diameter than human corneas (p < 0.001). Intermolecular spacing also varied with hydration in a bi-phasic manner but reached a maximum value at a lower hydration (H = 1.5) than fibril diameter. Human corneas displayed a higher intermolecular spacing than porcine corneas at all hydrations (p < 0.0001). Human and porcine corneas required a similar PEG concentration to reach physiological hydration, suggesting that the total fixed charge that gives rise to the swelling pressure is the same. The difference in their structural responses to hydration can be explained by variations in molecular crosslinking and intra/interfibrillar water partitioning.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Subjects: R Medicine > RE Ophthalmology
Uncontrolled Keywords: cornea, swelling, hydration, collagen, proteoglycans, fixed charge
Publisher: The Royal Society
ISSN: 1742-5689
Funders: Medical Research Council
Last Modified: 29 Mar 2018 20:32
URI: http://orca.cf.ac.uk/id/eprint/100684

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