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PAX6 isoforms, along with reprogramming factors, differentially regulate the induction of cornea-specific genes

Sasamoto, Yuzuru, Hayashi, Ryuhei, Park, Sung-Joon, Saito-Adachi, Mihoko, Suzuki, Yutaka, Kawasaki, Satoshi, Quantock, Andrew J, Nakai, Kenta, Tsujikawa, Motokazu and Nishida, Kohji 2016. PAX6 isoforms, along with reprogramming factors, differentially regulate the induction of cornea-specific genes. Scientific Reports 6 , 20807. 10.1038/srep20807

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Abstract

PAX6 is the key transcription factor involved in eye development in humans, but the differential functions of the two PAX6 isoforms, isoform-a and isoform-b, are largely unknown. To reveal their function in the corneal epithelium, PAX6 isoforms, along with reprogramming factors, were transduced into human non-ocular epithelial cells. Herein, we show that the two PAX6 isoforms differentially and cooperatively regulate the expression of genes specific to the structure and functions of the corneal epithelium, particularly keratin 3 (KRT3) and keratin 12 (KRT12). PAX6 isoform-a induced KRT3 expression by targeting its upstream region. KLF4 enhanced this induction. A combination of PAX6 isoform-b, KLF4 and OCT4 induced KRT12 expression. These new findings will contribute to furthering the understanding of the molecular basis of the corneal epithelium specific phenotype.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Optometry and Vision Sciences
Publisher: Nature Publishing Group
ISSN: 2045-2322
Date of First Compliant Deposit: 30 June 2017
Date of Acceptance: 8 January 2016
Last Modified: 17 Feb 2020 10:45
URI: http://orca.cf.ac.uk/id/eprint/101920

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