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Unfolding IGDQ peptides for engineering motogenic interfaces

De Leo, Federica, Marega, Riccardo, Corvaglia, Valentina, Tondo, Rodolfo, Lo Cicero, Matteo, Silvestrini, Simone and Bonifazi, Davide 2017. Unfolding IGDQ peptides for engineering motogenic interfaces. Langmuir 33 (30) , pp. 7512-7528. 10.1021/acs.langmuir.6b04381

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Abstract

Extracellular matrix (ECM)-mimicking surfaces are pivotal tools in understanding adherent cell physiopathology. In this sense, we have recently reported on a discrete set of ECM-mimicking SAMs, among which only those exposing IGDQ peptide-alkanethiols sustain the adhesion of MDA-MB-231 cells by triggering FAK phosphorylation and peculiarly induce the migration of individual cancer cells on the subcentimeter scale. Starting from the experimentally observed relationship among the SAM composition, organization, and biological response, a systematic computational characterization aided in pinpointing the atomistic details through which specific composition and organization achieve the desired biological responsiveness. Specifically, the solvent, number and type of peptides, and presence or absence of surface fillers were accurately considered, creating representative model SAMs simulated by means of classical molecular dynamics (MD) with a view toward unravelling the experimental evidence, revealing how the conformational and structural features of these substrates dictate the specific motogenic responses. Through complementary experimental and computational investigations, it clearly emerges that there exists a distinct and precise mutual interaction among IGDQ-peptides, the surface fillers, and Au, which controls the structural properties of the ECM-mimicking SAMs and thus their motogenic potential.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Uncontrolled Keywords: IGD peptides, molecular modeling, peptide SAMs, chemical gradient, cellular migration, cancer cells.
Publisher: ACS Publications
ISSN: 0743-7463
Funders: ERC Starting Grant
Date of First Compliant Deposit: 18 July 2017
Date of Acceptance: 6 July 2017
Last Modified: 03 Jul 2019 11:03
URI: http://orca.cf.ac.uk/id/eprint/102545

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