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HIF1α-associated circDENND4C promotes proliferation of breast cancer cells in hypoxic environment

Liang, Gehao, Liu, Zihao, Tan, Luyuan, Su, An, Jiang, Wen G. and Gong, Chang 2017. HIF1α-associated circDENND4C promotes proliferation of breast cancer cells in hypoxic environment. Anticancer Research 37 (8) , pp. 4337-4343. 10.21873/anticanres.11827

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Abstract

Background: Accumulating evidence has shown that hypoxia plays a key role in regulating proliferation of breast cancer cells. However, the mechanism of how hypoxia regulates breast cancer remains unclear. We sought to investigate if hypoxia regulated proliferation through circular RNA. Materials and Methods: Western blot was used to detect hypoxia-inducible factor 1 alpha (HIF1α) levels in breast cancer cells under hypoxic conditions. Candidate circular RNAs (circRNAs) were selected and quantified by quantitative real-time polymerase chain reaction (qRT-PCR) after hypoxia induction. CCK8 assay was used to investigate the changes of proliferation after interfering circDENND4C and HIF1a. Results: In breast cancer cells, circDENND4C was increased under hypoxic conditions and decreased after knocking-down HIF1α. In addition, knocking-down circDENND4C inhibited proliferation of breast cancer cells in a hypoxic environment. Finally, tumors with a large size had higher circDENND4C expression levels than those of small size. Conclusion: CircDENND4C is a HIF1α-associated circRNA promoting the proliferation of breast cancer cells under hypoxia.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: International Institute of Anticancer Research (IIAR)
ISSN: 0250-7005
Funders: National Natural Science Foundation of China, grant No. 81372138 and Cancer Research Wales
Date of First Compliant Deposit: 15 January 2018
Date of Acceptance: 31 May 2017
Last Modified: 22 Jan 2018 12:18
URI: http://orca.cf.ac.uk/id/eprint/103038

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