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Identification of islet antigen-specific CD8 T cells using MHCI-peptide tetramer reagents in the non obese diabetic (NOD) mouse model of type 1 diabetes

Pearson, James A. and Wong, Florence Susan 2015. Identification of islet antigen-specific CD8 T cells using MHCI-peptide tetramer reagents in the non obese diabetic (NOD) mouse model of type 1 diabetes. Methods in Molecular Biology 1433 , pp. 119-125. 10.1007/7651_2015_295

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Abstract

MHCI-peptide tetramer staining is an important technique in order to identify antigen-specific T cells within a heterogeneous cell population. The reagents may be used to isolate antigen-specific T cells and can help identify their role in disease. Here, we describe how to make tetramer from peptide:MHC monomers together with a protocol for staining antigen-specific cell populations with advice on generating a complementary antibody phenotyping panel.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Medicine
Uncontrolled Keywords: Antigen-specific; Flow cytometry; MHCI-peptide tetramer staining; T cell receptor.
Publisher: Springer
ISSN: 1064-3745
Last Modified: 02 Aug 2017 08:57
URI: http://orca.cf.ac.uk/id/eprint/103212

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