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KIAA1199 promotes migration and invasion by Wnt/β-catenin pathway and MMPs mediated EMT progression and serves as a poor prognosis marker in gastric cancer

Ahmad, Aamir, Jia, Shuqin, Qu, Tingting, Wang, Xiaohong, Feng, Mengmeng, Yang, Yang, Feng, Xuemin, Ma, Ruiting, Li, Wenmei, Hu, Ying, Feng, Yi, Ji, Ke, Li, Ziyu, Jiang, Wen and Ji, Jiafu 2017. KIAA1199 promotes migration and invasion by Wnt/β-catenin pathway and MMPs mediated EMT progression and serves as a poor prognosis marker in gastric cancer. PLoS ONE 12 (4) , e0175058. 10.1371/journal.pone.0175058

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Abstract

Background KIAA1199 was upregulated in diverse cancers, but the association of KIAA1199 with gastric cancer (GC), the biological role of KIAA1199 in GC cells and the related molecular mechanisms remain to be elucidated. Methods KIAA1199 expression was analysed by reverse transcription-polymerase chain reaction assay (RT-PCR) and immunohistochemistry (IHC) in GC patient tissue. The small hairpin RNA (shRNA) was applied for the knockdown of endogenous KIAA1199 in NCI-N87 and AGS cells. MTT, colony formation, scratch wounding migration, transwell chamber migration and invasion assays were employed respectively to investigate the role of KIAA1199 in GC cells. The potential signaling pathway of KIAA1199 induced migration and invasion was detected. Results KIAA1199 was upregulated in GC tissue and was an essential independent marker for poor prognosis. Knockdown KIAA1199 suppressed the proliferation, migration and invasion in GC cells. KIAA1199 stimulated the Wnt/β-catenin signaling pathway and the enzymatic activity of matrix metalloproteinase (MMP) family members and thus accelerated the epithelial-to-mesenchymal transition (EMT) progression in GC cells. Conclusion These findings demonstrated that KIAA1199 was upregulated in GC tissue and associated with worse clinical outcomes in GC, and KIAA1199 acted as an oncogene by promoting migration and invasion through the enhancement of Wnt/β-catenin signaling pathway and MMPs mediated EMT progression in GC cells

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Public Library of Science
ISSN: 1932-6203
Funders: Young Talents of Science and Technology Support Project of Colleges and Universities of Inner Mongolia Autonomous Region, the Great Project of the Affiliated Hospital of Inner Mongolia Medical University, National Natural Science Foundation of China, Beijing Municipal Administration of Hospitals’ Youth Programme, National Science and Technology Ministry, Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support
Date of First Compliant Deposit: 29 August 2017
Date of Acceptance: 20 March 2017
Last Modified: 22 May 2018 16:56
URI: http://orca.cf.ac.uk/id/eprint/103263

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