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Up-regulation of matrix metalloproteinase expression and activation following cyclical compressive loading of articular cartilage in vitro

Blain, Emma Jane, Gilbert, Sophie Jane, Wardale, Robert John, Capper, S. J., Mason, Deborah Jane and Duance, Victor Colin 2001. Up-regulation of matrix metalloproteinase expression and activation following cyclical compressive loading of articular cartilage in vitro. Archives of biochemistry and biophysics 396 (1) , pp. 49-55. 10.1006/abbi.2001.2575

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Abstract

Osteoarthritis (OA) results in articular cartilage degeneration and subchondral bone remodeling. Excessive or abnormal loading of the joint may contribute to matrix destruction by creating an imbalance between proteinases and their inhibitors. This study investigates whether cyclical loading regulates expression and/or activation of metalloproteinases 2 and 9 (MMPs) in articular cartilage explants. Gelatin zymography, reverse zymography, and MMP activity assays of mechanically loaded bovine cartilage explants (0.5 MPa, 1 Hz, 3 h) showed increased expression and activation of MMPs 2 and 9, whereas expression of the tissue inhibitors of MMPs was unaffected. This shows, for the first time that mechanical loading can influence tissue homeostasis generating an imbalance of proteinases and their inhibitors inducing turnover and/or catabolic events in cartilage.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QM Human anatomy
Uncontrolled Keywords: MMPs; TIMPs; articular cartilage; mechanical load
Publisher: Elsevier
ISSN: 0003-9861
Last Modified: 02 May 2019 11:03
URI: http://orca.cf.ac.uk/id/eprint/1044

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