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High-yield methods for accurate two-alternative visual psychophysics in head-fixed mice

Burgess, Christopher P., Lak, Armin, Steinmetz, Nicholas A., Zatka-Haas, Peter, Bai Reddy, Charu, Jacobs, Elina A.K., Linden, Jennifer F., Paton, Joseph J., Ranson, Adam, Schröder, Sylvia, Soares, Sofia, Wells, Miles J., Wool, Lauren E., Harris, Kenneth D. and Carandini, Matteo 2017. High-yield methods for accurate two-alternative visual psychophysics in head-fixed mice. Cell Reports 20 (10) , pp. 2513-2524. 10.1016/j.celrep.2017.08.047

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Abstract

Research in neuroscience increasingly relies on the mouse, a mammalian species that affords unparalleled genetic tractability and brain atlases. Here, we introduce high-yield methods for probing mouse visual decisions. Mice are head-fixed, facilitating repeatable visual stimulation, eye tracking, and brain access. They turn a steering wheel to make two alternative choices, forced or unforced. Learning is rapid thanks to intuitive coupling of stimuli to wheel position. The mouse decisions deliver high-quality psychometric curves for detection and discrimination and conform to the predictions of a simple probabilistic observer model. The task is readily paired with two-photon imaging of cortical activity. Optogenetic inactivation reveals that the task requires mice to use their visual cortex. Mice are motivated to perform the task by fluid reward or optogenetic stimulation of dopamine neurons. This stimulation elicits a larger number of trials and faster learning. These methods provide a platform to accurately probe mouse vision and its neural basis.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Neuroscience and Mental Health Research Institute (NMHRI)
Medicine
Subjects: R Medicine > R Medicine (General)
Additional Information: This is an open access article under the CCBY license
Publisher: Elsevier
ISSN: 2211-1247
Funders: Wellcome Trust
Date of First Compliant Deposit: 11 September 2017
Date of Acceptance: 14 August 2017
Last Modified: 19 Nov 2020 09:25
URI: http://orca.cf.ac.uk/id/eprint/104527

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