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Structural characterization of an α-1, 6-linked galactomannan from natural Cordyceps 2 sinensis

Wang, Junqiao, Nie, Shaoping, Chen, Shuping, Phillips, Aled O., Phillips, Glyn O., Li, Yajing, Xie, Mingyong and Cui, Steve W. 2018. Structural characterization of an α-1, 6-linked galactomannan from natural Cordyceps 2 sinensis. Food Hydrocolloids 78 , pp. 77-91. 10.1016/j.foodhyd.2017.07.024

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Abstract

An α-1, 6-linked galactomannan was isolated and purified from natural Cordyceps sinensis. The fine structure analysis of this polysaccharide was elucidated based on partial acid hydrolysis, monosaccharide composition, methylation and 1D/2D nuclear magnetic resonance (NMR) spectroscopy. Monosaccharide composition analysis revealed that this polysaccharide was mainly composed of galactose (68.65%), glucose (6.65%) and mannose (24.02%). However, after partial acid hydrolysis the percentages of galactose, glucose and mannose were changed to 3.96%, 13.82% and 82.22%, respectively. The molecular weight of this polysaccharide was 7207. Methylation and NMR analysis revealed that this galactomannan had a highly branched structure, mainly consisted of a mannan skeleton and galactofuranosyl chains. The structure of galactofuranosyl part was formed by alternating (1 → 5)-lined β-Galf and (1 → 6)-liked β-Galf or a single (1 → 6)-liked β-Galf, attaching to the O-2 and O-4 of the mannose chain, and terminated at β-T-Galf. The mannan core was revealed by analyzing the partial acid hydrolysate of the galactomannan and the structure was composed of (1 → 6)-linked α-Manp backbone, with substituted at C-2 by short chains of 2-substituted Manp or Galf branches.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Elsevier
ISSN: 0268-005X
Date of First Compliant Deposit: 22 September 2017
Date of Acceptance: 25 July 2017
Last Modified: 30 Jul 2018 10:07
URI: http://orca.cf.ac.uk/id/eprint/104949

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