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Telomere length heterogeneity in placenta revealed with high-resolution telomere length analysis

Garcia Martin, Isabel, Janssen, A.B., Jones, R.E., Grimstead, Julia W., Penketh, Richard J. A., Baird, Duncan M. and John, Rosalind M. 2017. Telomere length heterogeneity in placenta revealed with high-resolution telomere length analysis. Placenta 59 , pp. 61-68. 10.1016/j.placenta.2017.09.007

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Abstract

Introduction Telomeres, are composed of tandem repeat sequences located at the ends of chromosomes and are required to maintain genomic stability. Telomeres can become shorter due to cell division and specific lifestyle factors. Critically shortened telomeres are linked to cellular dysfunction, senescence and aging. A number of studies have used low resolution techniques to assess telomere length in the placenta. In this study, we applied Single Telomere Length Analysis (STELA) which provides high-resolution chromosome specific telomere length profiles to ask whether we could obtain more detailed information on the length of individual telomeres in the placenta. Methods Term placentas (37–42 weeks) were collected from women delivering at University Hospital of Wales or Royal Gwent Hospital within 2 h of delivery. Multiple telomere-length distributions were determined using STELA. Intraplacental variation of telomere length was analysed (N = 5). Telomere length distributions were compared between labouring (N = 10) and non-labouring (N = 11) participants. Finally, telomere length was compared between female (N = 17) and male (N = 20) placenta. Results There were no significant influences of sampling site, mode of delivery or foetal sex on the telomere-length distributions obtained. The mean telomere length was 7.7 kb ranging from 5.0 kb to 11.7 kb across all samples (N = 42) and longer compared with other human tissues at birth. STELA also revealed considerable telomere length heterogeneity within samples. Conclusions We have shown that STELA can be used to study telomere length homeostasis in the placenta regardless of sampling site, mode of delivery and foetal sex. Moreover, as each amplicon is derived from a single telomeric molecule, from a single cell, STELA can reveal the full detail of telomere-length distributions, including telomeres within the length ranges observed in senescent cells. STELA thus provides a new tool to interrogate the relationship between telomere length and pregnancy complications linked to placental dysfunction.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Medicine
Publisher: Elsevier
ISSN: 0143-4004
Date of First Compliant Deposit: 26 September 2017
Date of Acceptance: 18 September 2017
Last Modified: 25 Feb 2019 23:33
URI: http://orca.cf.ac.uk/id/eprint/104987

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