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Hyperglycaemia does not affect antigen-specific activation and cytolytic killing by CD8+ T cells in vivo

Recino, Asha, Barkan, Kerry, Wong, F. Susan, Ladds, Graham, Cooke, Anne and Wallberg, Maja 2017. Hyperglycaemia does not affect antigen-specific activation and cytolytic killing by CD8+ T cells in vivo. Bioscience Reports 37 (4) , 1079. 10.1042/BSR20171079

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Abstract

Metabolism is of central importance for T cell survival and differentiation. It is well known that T cells cannot function in the absence of glucose, but it is less clear how they respond to excessive levels of glucose. In the present study, we investigated how increasing levels of glucose affect T-cell-mediated immune responses. We examined the effects of increased levels of glucose on CD8+ T-cell behaviour in vitro by assessing activation and cytokine production, as well as oxygen consumption rate (OCR), extracellular acidification rate (ECAR) and intracellular signalling. In addition, we assessed in vivo proliferation, cytokine production and cytolytic activity of cells in chemically induced diabetic C57BL/6 mice. Elevated levels of glucose in in vitro cultures had modest effects on proliferation and cytokine production, while in vivo hyperglycaemia had no effect on CD8+ T-cell proliferation, interferon γ (IFNγ) production or cytolytic killing.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Portland Press
ISSN: 0144-8463
Date of First Compliant Deposit: 9 October 2017
Date of Acceptance: 9 August 2017
Last Modified: 09 Oct 2017 09:53
URI: http://orca.cf.ac.uk/id/eprint/105268

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