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Molecular genotyping of bacillus anthracis strains from Georgia and northeastern part of Turkey

Khmaladze, E, Su, W, Zghenti, E, Buyuk, F, Sahin, M, Nicolich,MP, MP, Baillie, Les, Obiso, R and Kotorashvili, A 2017. Molecular genotyping of bacillus anthracis strains from Georgia and northeastern part of Turkey. Journal of Bacteriology and Mycology 4 (3)

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Abstract

Bacillus anthracis is the causal agent of anthrax and has a history of use as a biological weapon. Anthrax cases occur worldwide and the disease is endemic in certain regions. Here we describe a study of the genetic diversity of B. anthracis strains in two endemic areas: The country of Georgia and the Kars region of Turkey. Thirty Turkish isolates and thirty Georgian isolates were subjected to Single Nucleotide Polymorphism (SNP) sub typing, followed by higher-resolution genotyping using 25-loci variable-number tandem repeat analysis (MLVA-25). Canonical SNP typing indicated that Turkish strains belonged to both the A.Br.003 linage and the Australian 94 lineage. In light of a recent analysis that placed the majority of Georgian B. anthracis isolates in one phylogenetic group, we screened the Turkish strains using a previously developed Georgian SNP panel. Minimal diversity was observed among the Kars strains within the Georgian SNP lineage: all 30 of these strains grouped with A.Br.026, ten strains were derived from A.Br.028, and only two isolates belonged to A.Br.029. According to the results of MLVA-25 genotyping, all 30 Turkish strains belong to two clusters. Cluster A is more diverse than cluster B. Our results suggest that B. anthracis strains originating from Georgia and the northeastern part of Turkey are genetically interrelated, which could be explained by the geographic proximity of the countries.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Pharmacy
Publisher: Austin Publishing Group
ISSN: 2471-0172
Date of First Compliant Deposit: 13 October 2017
Date of Acceptance: 30 August 2017
Last Modified: 17 Oct 2017 10:28
URI: http://orca.cf.ac.uk/id/eprint/105523

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