Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Site-specific one-to-one click coupling of single proteins to individual carbon nanotubes: a single-molecule approach

Freeley, Mark, Worthy, Harley L., Ahmed, Rochelle, Bowen, Benjamin, Watkins, Daniel, MacDonald, John, Zheng, Ming, Jones, Darran Dafydd and Palma, Matteo 2017. Site-specific one-to-one click coupling of single proteins to individual carbon nanotubes: a single-molecule approach. Journal of the American Chemical Society 139 (49) , pp. 17834-17840. 10.1021/jacs.7b07362

[img]
Preview
PDF - Accepted Post-Print Version
Download (4MB) | Preview

Abstract

We report the site-specific coupling of single proteins to individual carbon nanotubes (CNTs) in solution and with singlemolecule control. Using an orthogonal Click reaction, Green Fluorescent Protein (GFP) was engineered to contain a genetically encoded azide group and then bound to CNT ends in different configurations: in close proximity or at longer distances from the GFP’s functional centre. Atomic force microscopy and fluorescence analysis in solution and on surfaces at the single-protein level confirmed the importance of bioengineering optimal protein attachment sites to achieve direct protein-nanotube communication and bridging.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Physics and Astronomy
Subjects: Q Science > QC Physics
Publisher: American Chemical Society
ISSN: 0002-7863
Date of First Compliant Deposit: 22 November 2017
Date of Acceptance: 16 November 2017
Last Modified: 28 Nov 2018 22:16
URI: http://orca.cf.ac.uk/id/eprint/106939

Citation Data

Cited 2 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics