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Mucosal immunisation of murine neonates using whole cell and acellular Pertussis vaccines

Hale, C, Humphreys, Ian, Hussell, T, Bowe, F, Clare, S, Pickard, D, Preston, A, Del Giudice, G and Dougan, G 2004. Mucosal immunisation of murine neonates using whole cell and acellular Pertussis vaccines. Vaccine 22 (27-28) , pp. 3595-3602. 10.1016/j.vaccine.2004.03.039

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Abstract

Groups of neonatal mice were immunised with different mucosal vaccines based on acellular (Pertactin antigen) or whole cell (inactivated Bordetella pertussis with Diphtheria and Tetanus toxoid) Pertussis vaccines, using Escherichia coli heat-labile enterotoxin (LT) as a mucosal adjuvant. Neonatal mice tolerated mucosal vaccination well and a significant cellular infiltrate was detected in the lungs of mice receiving mucosal vaccines compared to PBS controls. This infiltrate included B lymphocytes, gammadelta T cells and interferon-gamma producing T cells. Neonatal mice, in contrast to adult mice, responded poorly in terms of the production of serum antibody to Pertussis antigens delivered mucosally, although they were able to mount an anti-Tetanus response to those vaccines harbouring Tetanus toxoid and whole cell Pertussis antigen. Neonatal mice immunised with Pertactin or whole cell Pertussis antigen together with LT were protected against virulent B. pertussis challenge

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: Aging/physiology;Animals;Animals, Newborn/physiology*;Antibody Formation/immunology;Antigens, Surface/analysis;Bordetella pertussis/immunology;Bronchoalveolar Lavage;Fluid/microbiology;Cell Division/physiology;Colony Count, Microbial;Diphtheria-Tetanus-Pertussis Vaccine/immunology;Enzyme-Linked Immunosorbent Assay;Female; Flow Cytometry;Immunity, Mucosal/immunology*;Indicators and Reagents;Lung/microbiology;Mice;Mice, Inbred BALB C;Pertussis Vaccine/therapeutic use*;T-Lymphocytes/immunology;Vaccines, Acellular/therapeutic use Whooping Cough/immunology;Whooping Cough/microbiology;Whooping Cough/prevention & control*;
Publisher: Elsevier
ISSN: 0264-410X
Date of Acceptance: 27 March 2004
Last Modified: 05 Dec 2017 02:57
URI: http://orca.cf.ac.uk/id/eprint/107190

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