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Short peptide analogs as alternatives to collagen in pro-regenerative corneal implants

Jangamreddy, J. R., Haagdorens, M., Islam, M. M., Lewis, Philip, Samanta, A., Fagerholm, P., Liszka, A., Ljunggren, M. K., Buznyk, O., Alarcon, E., Zakaria, N., Meek, Keith Michael Andrew and Griffith, M. 2018. Short peptide analogs as alternatives to collagen in pro-regenerative corneal implants. Acta Biomaterialia 69 , pp. 120-130. 10.1016/j.actbio.2018.01.011

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Abstract

Short collagen-like peptides (CLPs) are being proposed as alternatives to full-length collagen for use in tissue engineering, on their own as soft hydrogels, or conjugated to synthetic polymer for mechanical strength. However, despite intended clinical use, little is known about their safety and efficacy, mechanism of action or degree of similarity to the full-length counterparts they mimic. Here, we show the functional equivalence of a CLP conjugated to polyethylene glycol (CLP-PEG) to full-length recombinant human collagen in vitro and in promoting stable regeneration of corneal tissue and nerves in a pre-clinical mini-pig model. We also show that these peptide analogs exerted their pro-regeneration effects through stimulating extracellular vesicle production by host cells. Our results support future use of CLP-PEG implants for corneal regeneration, suggesting the feasibility of these or similar peptide analogs in clinical application in the eye and other tissues.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Publisher: Elsevier
ISSN: 1742-7061
Funders: MRC
Date of First Compliant Deposit: 16 January 2018
Date of Acceptance: 10 January 2018
Last Modified: 03 Apr 2019 12:54
URI: http://orca.cf.ac.uk/id/eprint/108212

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