Nitric oxide synthase in cultured endocardial cells of the pig

Schulz, R., Warden-Smith, Jeremy, Lewis, Malcolm John and Moncada, S. 1991. Nitric oxide synthase in cultured endocardial cells of the pig. British Journal of Pharmacology 104 (1) , pp. 21-24.

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Abstract

1. Endocardial cells release factors which regulate myocardial contractility and guanosine 3':5'-cyclic monophosphate (cyclic GMP) levels. One of these factors is indistinguishable from endothelium-derived relaxing factor (EDRF). 2. The effluent from pig heart endocardial cells cultured on microcarrier beads caused the relaxation of a pig coronary artery ring denuded of endothelium. This relaxation was enhanced by a combination of superoxide dismutase and catalase and was attenuated by haemoglobin, which binds nitric oxide (NO), and by inhibitors of NO synthase, NG-monomethyl-L-arginine (L-NMMA) or NG-nitro-L-arginine. 3. A Ca(2+)-, L-arginine- and NADPH-dependent enzyme activity which generated NO was detected by a specific spectrophotometric assay in cytosol prepared from endocardial cells. The formation of NO was inhibited in a concentration-dependent manner by L-NMMA (but not D-NMMA) and this could be partially reversed upon addition of excess L-arginine. 4. Like endothelial cells from the blood vessels, the endocardial cells possess the ability to synthesize NO, which may act to regulate myocardial contractility.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Medicine Pharmacy
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords:	Endocardium ; Endothelium-derived relaxing factor (EDRF); Nitric oxide ; Cell culture
Publisher:	Wiley-Blackwell
ISSN:	0007-1188
Last Modified:	17 Mar 2021 02:34
URI:	https://orca.cardiff.ac.uk/id/eprint/10866

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