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Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

Zaki, Athraa, Hartley, Andrew, Reddington, Samuel, Thomas, Suzanne, Watson, Peter, Hayes, Anthony, Moskalenko, Andy, Craciun, Monica, MacDonald, John, Jones, Darran Dafydd and Elliott, Martin 2018. Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle. RSC Advances 8 , pp. 5768-5775. 10.1039/c7ra11166e

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Abstract

We have created modified protein variants by introducing a non-canonical amino acid p-azido-Lphenylalanine (azF) into defined positions for photochemically-induced covalent attachment to graphene. Attachment of GFP, TEM and cyt b562 proteins was verified through a combination of atomic force and scanning tunnelling microscopy, resistance measurements, Raman data and fluorescence measurements. This method can in principle be extended to any protein which can be engineered in this way without adversely affecting its structural stability

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Physics and Astronomy
Publisher: RSC Publishing
ISSN: 2046-2069
Funders: EPSRC
Date of First Compliant Deposit: 27 February 2018
Date of Acceptance: 11 January 2018
Last Modified: 29 Jan 2019 22:28
URI: http://orca.cf.ac.uk/id/eprint/109591

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