Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

XPF plays an indispensable role in relieving silver nanoparticle induced DNA damage stress in human cells

Wang, Dan, Yang, Huali, Zhou, Zheng, Zhao, Man, Chen, Runsheng and Reed, Simon 2018. XPF plays an indispensable role in relieving silver nanoparticle induced DNA damage stress in human cells. Toxicology Letters 288 , pp. 44-54. 10.1016/j.toxlet.2018.02.022

Full text not available from this repository.

Abstract

Due to the specific antimicrobial activity of silver nanoparticles (AgNPs), they are widely used in wound dressings, coatings in medical devices and household products. In spite of the well-documented genotoxicity of AgNPs, the molecular mechanisms of relieving AgNP-induced DNA damage stress remain poorly understood. We report here that one of the DNA repair factors, XPF, plays a crucial role in resisting AgNP-induced DNA damage stress in human cells. Following culture with AgNP-containing media, severely decreased colony forming abilities have been observed in XPF mutant and knockdown cells compared with wild type or control cells respectively, demonstrating that XPF is required to resist the AgNP-induced stress. By employing the comet assays, we confirmed that DNA damages were produced in all tested cells following their exposure to AgNPs for 48 h. However, more DNA damage accumulations were observed in XPF mutant and knockdown cells than wild type or control cells respectively. Moreover, severe DNA damage response and the activation of p53-mediated DNA damage response network result from mutated XPF or significantly reduced XPF level in human cells. Together, our results illustrate that XPF is the indispensable factor involved in relieving AgNP-induced DNA damage stress in human cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Elsevier
ISSN: 0378-4274
Date of First Compliant Deposit: 9 March 2018
Date of Acceptance: 13 February 2018
Last Modified: 12 Mar 2018 15:29
URI: http://orca.cf.ac.uk/id/eprint/109794

Citation Data

Cited 1 time in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item