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Quantification of human complement C2 protein using an automated turbidimetric immunoassay

Tange, Clare Elizabeth, Johnson-Brett, Bridget, Cook, Alex, Stordeur, Patrick, Brohet, Fabian, Jolles, Stephen, Steven, Rachel, Ponsford, Mark, Roberts, Andrew, El-Shanawany, Tariq, Harding, Stephen, Wallis, Gregg and Parker, Antony Richard 2018. Quantification of human complement C2 protein using an automated turbidimetric immunoassay. Clinical Chemistry and Laboratory Medicine 56 (9) , pp. 1498-1506. 10.1515/cclm-2017-1068

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Abstract

Background: The measurement of complement components is clinically useful where a deficiency is suspected, or where excessive activation and consumption are present in disease. C2 deficiency carries an increased risk of developing systemic lupus erythematosus, recurrent infections and atherosclerosis. In this study, we have evaluated The Binding Site’s Human Complement C2 SPAPLUS® assay. Methods: Linearity was tested using 13 sample dilutions covering the standard measuring range. Within- and between-assay variabilities were calculated using five samples with different C2 concentrations. The correlation between C2 concentrations in EDTA-plasma and serum was assessed, as was the correlation between C2 measurements by the automated assay and radial immunodiffusion. C2 concentrations were compared with CH50 activity, and quantified in individuals with homozygous or heterozygous C2 deficiency, acquired angioedema and patients with chronic inflammatory conditions. Results: The assay was linear across the measuring range (3.8–42.3 mg/L). Intra- and interassay variability were 2.3%–3.8% and 0%–3.3%, respectively. Comparison between C2 measurements in EDTA-plasma and serum provided a strong correlation (p<0.0001, R2=0.82, slope 0.92), as did the correlation between the automated and radial immunodiffusion methods (p<0.0001, R2=0.89, slope 1.07). A positive correlation between C2 concentration and CH50 activity was demonstrated (p<0.0001, R2=0.48). Significant differences were observed between the median C2 concentrations obtained in healthy controls and the patient clinical samples, with homozygous C2-deficient patients giving below detectable results. Conclusions: This C2 SPAPLUS® assay allows the automated, rapid and precice quantification of complement C2 protein and could therefore be considered as a replacement for older, more time-consuming methods.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Medicine
Publisher: Walter de Gruyter
ISSN: 1434-6621
Date of First Compliant Deposit: 7 June 2018
Date of Acceptance: 9 March 2018
Last Modified: 28 Apr 2019 12:46
URI: http://orca.cf.ac.uk/id/eprint/111568

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