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Time-lapse ultrashort pulse microscopy of infection in three-dimensional versus two-dimensional culture environments reveals enhanced extra-chromosomal virus replication compartment formation

Gibbs, Holly C., Sing, Garwin, Armas, Juan Carlos González, Campbell, Colin J., Ghazal, Peter and Yeh, Alvin T. 2013. Time-lapse ultrashort pulse microscopy of infection in three-dimensional versus two-dimensional culture environments reveals enhanced extra-chromosomal virus replication compartment formation. Journal of Biomedical Optics 18 (3) , 031111. 10.1117/1.JBO.18.3.031111

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Abstract

The mechanisms that enable viruses to harness cellular machinery for their own survival are primarily studied in cell lines cultured in two-dimensional (2-D) environments. However, there are increasing reports of biological differences between cells cultured in 2-D versus three-dimensional (3-D) environments. Here we report differences in host-virus interactions based on differences in culture environment. Using ultrashort pulse microscopy (UPM), a form of two-photon microscopy that utilizes sub-10-fs pulses to efficiently excite fluorophores, we have shown that de novo development of extra-chromosomal virus replication compartments (VRCs) upon murine cytomegalovirus (mCMV) infection is markedly enhanced when host cells are cultured in 3-D collagen gels versus 2-D monolayers. In addition, time-lapse imaging revealed that mCMV-induced VRCs have the capacity to grow by coalescence. This work supports the future potential of 3-D culture as a useful bridge between traditional monolayer cultures and animal models to study host-virus interactions in a more physiologically relevant environment for the development of effective anti-viral therapeutics. These advances will require broader adoption of modalities, such as UPM, to image deep within scattering tissues.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Medicine
Publisher: Society of Photo-optical Instrumentation Engineers (SPIE)
ISSN: 1083-3668
Date of Acceptance: 18 January 2013
Last Modified: 21 Jun 2018 13:16
URI: http://orca.cf.ac.uk/id/eprint/112266

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