Influence of vitamin D status and mechanical loading on the morphometric and mechanical properties of the mouse tibia

Lu, Yong-Tao, Cui, Zhen-Tao, Zhu, Han-Xing ORCID: https://orcid.org/0000-0002-3209-6831, Ma, Ru-Kun and Wu, Cheng-Wei 2019. Influence of vitamin D status and mechanical loading on the morphometric and mechanical properties of the mouse tibia. Journal of Medical and Biological Engineering 39 (4) , pp. 523-531. 10.1007/s40846-018-0433-7

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Abstract

It is well known that vitamin D and mechanical loading play important roles in bone growth and development. However, the combined effect of the maternal vitamin D status and mechanical loading on the bone quality of growing and mature bones is still unclear. The aim of this study was to investigate the influence of the antenatal vitamin D status and mechanical loading on bone morphometric and mechanical properties in juvenile and adult bones. C57BL/6J mice were used to generate vitamin D-replete and vitamin D-depleted dams. The left tibiae of 8-week-old and 16-week-old offspring were mechanically loaded in vivo for two weeks. Both tibiae were dissected and scanned using a μCT imaging system. It was found that in the bones of 10-week-old juvenile offspring, the antenatal vitamin D-replete group significantly increased trabecular bone volume fraction (Tb.BV/TV), trabecular thickness (Tb.Th), cortical thickness (Ct.Th), bone stiffness and failure load; significantly decreased trabecular separation (Tb.Sp) and cortical marrow area (Ct.MA) only in loaded tibiae; and markedly increased Tb.Sp and Ct.MA only in non-loaded tibiae. In the bones of the 18-week-old adult offspring, the antenatal vitamin D status had a minimal effect on the bone morphometric and mechanical parameters. These data imply that antenatal vitamin D repletion results in increased responses to mechanical loading only in the juvenile state, emphasizing the importance of a sufficient vitamin D supply during pregnancy and sufficient physical activities during the juvenile period to increase bone quality.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Engineering
Publisher:	Springer
ISSN:	1609-0985
Date of First Compliant Deposit:	18 June 2018
Date of Acceptance:	5 June 2018
Last Modified:	06 Nov 2023 18:17
URI:	https://orca.cardiff.ac.uk/id/eprint/112550

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