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Development of immunosensors for direct detection of three wound infection biomarkers at point of care using electrochemical impedance spectroscopy

Ciani, Ilenia, Schulze, Holger, Corrigan, Damion K., Henihan, Grace, Giraud, Gerard, Terry, Jonathan G., Walton, Anthony J., Pethig, Ronald, Ghazal, Peter, Crain, Jason, Campbell, Colin J., Bachmann, Till T. and Mount, Andrew R. 2012. Development of immunosensors for direct detection of three wound infection biomarkers at point of care using electrochemical impedance spectroscopy. Biosensors and Bioelectronics 31 (1) , pp. 413-418. 10.1016/j.bios.2011.11.004

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Abstract

A method for label-free, electrochemical impedance immunosensing for the detection and quantification of three infection biomarkers in both buffer and directly in the defined model matrix of mock wound fluid is demonstrated. Triggering Receptor-1 Expressed on Myeloid cells (TREM-1) and Matrix MetalloPeptidase 9 (MMP-9) are detected via direct assay and N-3-oxo-dodecanoyl-l-HomoSerineLactone (HSL), relevant in bacterial quorum sensing, is detected using a competition assay. Detection is performed with gold screen-printed electrodes modified with a specific thiolated antibody. Detection is achieved in less than 1h straight from mock wound fluid without any extensive sample preparation steps. The limits of detection of 3.3 pM for TREM-1, 1.1 nM for MMP-9 and 1.4 nM for HSL are either near or below the threshold required to indicate infection. A relatively large dynamic range for sensor response is also found, consistent with interaction between neighbouring antibody-antigen complexes in the close-packed surface layer. Together, these three novel electrochemical immunosensors demonstrate viable multi-parameter sensing with the required sensitivity for rapid wound infection detection directly from a clinically relevant specimen.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Elsevier
ISSN: 0956-5663
Last Modified: 31 Jan 2020 04:20
URI: http://orca.cf.ac.uk/id/eprint/112586

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