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Heterodyne dual-polarization epi-detected CARS microscopy for chemical and topographic imaging of interfaces

Langbein, Wolfgang, Regan, David, Pope, Iestyn and Borri, Paola 2018. Heterodyne dual-polarization epi-detected CARS microscopy for chemical and topographic imaging of interfaces. APL Photonics
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Abstract

Coherent Raman Scattering (CRS) has emerged in the last decade as a powerful multiphoton microscopy technique o�ering chemically-speci�c label-free imaging in real time with high three-dimensional spatial resolution. Many technical realizations of CRS microscopy have been proposed to remove, suppress, or account for the non-resonant background in the nonlinear susceptibility which complicates spectral analysis and reduces image contrast. Here, we demonstrate coherent anti-Stokes Raman scattering microscopy using a dual-polarization balanced heterodyne detection in epi-geometry (eH-CARS), providing background-free chemically-speci�c image contrast for nanoparticles and interfaces, shot-noise limited detection, and phase sensitivity. We show the sensitivity and selectivity of eH-CARS in comparison with forward CARS and stimulated Raman scattering (SRS) on polystyrene beads in agarose gel. As an important biologically-relevant application, we demonstrate eH-CARS imaging of individual lipid bilayers with high contrast and topographic sensitivity.

Item Type: Article
Date Type: Published Online
Status: In Press
Schools: Physics and Astronomy
Subjects: Q Science > QC Physics
Publisher: AIP Publishing
ISSN: 2378-0967
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Date of First Compliant Deposit: 6 July 2018
Date of Acceptance: 22 June 2018
Last Modified: 31 Oct 2018 20:20
URI: http://orca.cf.ac.uk/id/eprint/113008

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