Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Switchable genome editing via genetic code expansion

Suzuki, Toru, Asami, Maki, Patel, Sanjay G., Luk, Louis Y. P., Tsai, Yu-Hsuan and Perry, Anthony C. F. 2018. Switchable genome editing via genetic code expansion. Scientific Reports 8 (1) , 10051. 10.1038/s41598-018-28178-3

[img]
Preview
PDF - Published Version
Available under License Creative Commons Attribution.

Download (5MB) | Preview

Abstract

Multiple applications of genome editing by CRISPR-Cas9 necessitate stringent regulation and Cas9 variants have accordingly been generated whose activity responds to small ligands, temperature or light. However, these approaches are often impracticable, for example in clinical therapeutic genome editing in situ or gene drives in which environmentally-compatible control is paramount. With this in mind, we have developed heritable Cas9-mediated mammalian genome editing that is acutely controlled by the cheap lysine derivative, Lys(Boc) (BOC). Genetic code expansion permitted non-physiological BOC incorporation such that Cas9 (Cas9BOC) was expressed in a full-length, active form in cultured somatic cells only after BOC exposure. Stringently BOC-dependent, heritable editing of transgenic and native genomic loci occurred when Cas9BOC was expressed at the onset of mouse embryonic development from cRNA or Cas9BOC transgenic females. The tightly controlled Cas9 editing system reported here promises to have broad applications and is a first step towards purposed, spatiotemporal gene drive regulation over large geographical ranges.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Publisher: Nature Publishing Group
ISSN: 2045-2322
Date of First Compliant Deposit: 11 July 2018
Date of Acceptance: 11 June 2018
Last Modified: 12 Jul 2018 10:16
URI: http://orca.cf.ac.uk/id/eprint/113141

Citation Data

Cited 1 time in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics