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Evidence for adipocyte-derived extracellular vesicles in the human circulation

Connolly, Katherine D., Wadey, Rebecca M., Mathew, Donna, Johnson, Errin, Rees, D. Aled and James, Philip E.. 2018. Evidence for adipocyte-derived extracellular vesicles in the human circulation. Endocrinology 159 (9) , pp. 3259-3267. 10.1210/en.2018-00266
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Abstract

Adipocyte-derived extracellular vesicles (EVs) may serve as novel endocrine mediators of adipose tissue and impact upon vascular health. However, it is unclear whether adipocyte-derived EVs are present in the human circulation. Therefore, the purpose of this study was to seek evidence for the presence of adipocyte-derived EVs in circulating plasma. Size exclusion chromatography of platelet-free plasma identified fractions 5-10 as containing EVs by a peak in particle concentration, which corresponded with the presence of EV and adipocyte proteins. Pooling fractions 5-10 and subjecting to ultracentrifugation yielded a plasma EV sample, as verified by transmission electron microscopy (TEM) showing EV structures and Western blotting for EV (e.g. CD9 and Alix) and adipocyte markers. Magnetic beads and a solid phase assay were used to deplete the EV sample of the four major families of circulating EVs: platelet-, leukocyte-, endothelial- and erythrocyte-derived EVs. Post-depletion samples from both techniques contained EV structures as visualized by TEM, as well as CD9, Alix and classic adipocyte proteins. Post-depletion samples also contained a range of other adipocyte proteins from an adipokine array. Adipocyte proteins and adipokines are expressed in optimally processed plasma EV samples, suggesting that adipocyte-derived EVs are secreted into the human circulation.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG)
Publisher: Oxford University Press
ISSN: 0013-7227
Date of First Compliant Deposit: 12 July 2018
Date of Acceptance: 10 July 2018
Last Modified: 01 Sep 2018 12:21
URI: http://orca.cf.ac.uk/id/eprint/113164

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