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Fluorescence lifetime imaging of quantum dot labeled DNA microarrays

Giraud, Gerard, Schulze, Holger, Bachmann, Till, Campbell, Colin, Mount, Andrew, Ghazal, Peter, Khondoker, Mizanur, Ross, Alan, Ember, Stuart, Ciani, Ilenia, Tlili, Chaker, Walton, Anthony, Terry, Jonathan and Crain, Jason 2009. Fluorescence lifetime imaging of quantum dot labeled DNA microarrays. International Journal of Molecular Sciences 10 (4) , pp. 1930-1941. 10.3390/ijms10041930

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Abstract

Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime value of 18.8 ns, compared to 13.3 ns obtained for spots of free QD solution, revealing that QD labels are sensitive to the spot microenvironment. Additionally, time gated detection was shown to improve the microarray image contrast ratio by 1.8, achieving femtomolar target sensitivity. Finally, lifetime multiplexing based on Qdot525 and Alexa430 was demonstrated using a single excitation-detection readout channel.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: MDPI
ISSN: 1422-0067
Date of First Compliant Deposit: 6 December 2018
Date of Acceptance: 21 April 2009
Last Modified: 06 Dec 2018 17:00
URI: http://orca.cf.ac.uk/id/eprint/113275

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