Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

DNA Nanoswitch as a Biosensor

Buck, Amy H., Campbell, Colin J., Dickinson, Paul, Mountford, Christopher P., Stoquert, Hélène C., Terry, Jonathan G., Evans, Stuart A. G., Keane, Lorraine M., Su, Tsueu-Ju, Mount, Andrew R., Walton, Anthony J., Beattie, John S., Crain, Jason and Ghazal, Peter 2007. DNA Nanoswitch as a Biosensor. Analytical Chemistry 79 (12) , pp. 4724-4728. 10.1021/ac070251r

Full text not available from this repository.

Abstract

We present a new type of DNA switch, based on the Holliday junction, that uses a combination of binding and conformational switching to enable specific label-free detection of DNA and RNA. We show that a single RNA oligonucleotide species can be detected in a complex mixture of extracted cellular RNA and demonstrate that by exploiting different aspects of the switch characteristics we can achieve 30-fold discrimination between single-nucleotide mismatches in a DNA oligonucleotide.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: American Chemical Society
ISSN: 0003-2700
Last Modified: 20 Jul 2018 13:15
URI: http://orca.cf.ac.uk/id/eprint/113314

Citation Data

Cited 18 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item