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Positional characteristics of fluorophores influencing signal output of a DNA nanoswitch

Dickinson, Paul, Campbell, Colin, Evans, Stuart G, Buck, Amy, Mountford, Christopher, Keane, Lorraine, Terry, Jonathan, Su, Tsueu-ju, Mount, Andrew, Walton, Anthony, Beattie, John, Crain, Jason and Ghazal, Peter 2006. Positional characteristics of fluorophores influencing signal output of a DNA nanoswitch. Presented at: Bio Micro and Nanosystems Conference, San Francisco, California, 15-18 January 2006. Bio Micro and Nanosystems Conference 2006. United States: Institute of Electrical & Electronics Engineers (IEEE) Inc, p. 3. 10.1109/BMN.2006.330892

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Abstract

The Holliday junction (HJ) structure, consisting of four DNA double helices with a central branch point, is capable of switching between conformational states upon ion binding. The HJ nanoswitch described here comprises a long, dual labeled cloverleaf oligonucleotide and a short, unlabeled oligonucleotide. Fluorescent labeling with donor and acceptor dyes placed on the HJ arms of the cloverleaf strand allows the ion induced conformational switch to be detected optically using fluorescence resonance energy transfer (FRET). The influence of donor and acceptor dye location on the detection of conformational switching has been investigated using two distinct HJ structures. In addition, the effect of increasing HJ arm length in order to increase donor and acceptor dye separation has been evaluated. We report that a preferential HJ nanoswitch structure can be determined, capable of efficient detection of ion induced conformational switching

Item Type: Conference or Workshop Item (Paper)
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Institute of Electrical & Electronics Engineers (IEEE) Inc
ISBN: 978-1424400560
Last Modified: 20 Aug 2018 11:00
URI: http://orca.cf.ac.uk/id/eprint/113392

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