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Development of non-agglutination microarray blood grouping

Robb, J. S., Roy, D. J., Ghazal, P., Allan, J. and Petrik, J. 2006. Development of non-agglutination microarray blood grouping. Transfusion Medicine 16 (2) , pp. 119-129. 10.1111/j.1365-3148.2005.00628.x

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Abstract

Varicella-zoster virus (VZV) is a human herpes virus that causes varicella as a primary infection and herpes zoster following reactivation of the virus from a latent state in trigeminal and spinal ganglia. In order to study the global pattern of VZV gene transcription, VZV microarrays using 75-base oligomers to 71 VZV open reading frames (ORFs) were designed and validated. The long-oligonucleotide approach maximizes the stringency of detection and polarity of gene expression. To optimize sensitivity, microarrays were hybridized to target RNA and the extent of hybridization measured using resonance light scattering. Microarray data were normalized to a subset of invariant ranked host-encoded positive-control genes and the data subjected to robust formal statistical analysis. The programme of viral gene expression was determined for VZV (Dumas strain)-infected MeWocells and SVG cells (an immortalized human astrocyte cell line) 72 h post-infection. Marked quantitative and qualitative differences in the viral transcriptome were observed between the two different cell types using the Dumas laboratory-adapted strain. Oligonucleotide-based VZV arrays have considerable promise as a valuable tool in the analysis of viral gene transcription during both lytic and latent infections, and the observed heterogeneity in the global pattern of viral gene transcription may also have diagnostic potential

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Wiley: 12 months
ISSN: 0958-7578
Date of Acceptance: 22 July 2005
Last Modified: 25 Jul 2018 13:45
URI: http://orca.cf.ac.uk/id/eprint/113401

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