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Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos

FitzHarris, Greg, Carroll, John and Swann, Karl 2018. Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos. Methods in Cell Biology 144 , pp. 431-440. 10.1016/bs.mcb.2018.03.036

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Abstract

Microinjection is an essential approach in the study of mammalian oocytes and early embryos, and is useful for the introduction of many molecules and reagents. Whereas microinjection into germinal vesicle stage oocytes is relatively simple using various microinjection setups, metaphase-II mouse eggs are notoriously fragile, and nondamaging microinjection can be difficult to achieve. Here we describe a microinjection method that is based on electrophysiology, which vastly reduces microinjection damage, especially in metaphase-II eggs. When optimized, this approach allows for over 90% oocyte survival, increasing confidence in experimental results

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Biosciences
Publisher: ELSEVIER ACADEMIC PRESS INC
ISSN: 0091-679X
Date of First Compliant Deposit: 3 August 2018
Date of Acceptance: 24 May 2018
Last Modified: 02 May 2019 11:35
URI: http://orca.cf.ac.uk/id/eprint/113881

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