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Neocortical long-term potentiation and experience-dependent synaptic plasticity require alpha-calcium/calmodulin-dependent protein kinase II autophosphorylation

Hardingham, Neil Robert, Glazewski, Stanislaw, Pakhotin, Pavel, Mizuno, Keiko, Chapman, Paul Frederick James, Giese, K. Peter and Fox, Kevin Dyson 2003. Neocortical long-term potentiation and experience-dependent synaptic plasticity require alpha-calcium/calmodulin-dependent protein kinase II autophosphorylation. Journal of Neuroscience 23 (11) , pp. 4428-4436.

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Abstract

Experience-dependent plasticity can be induced in the barrel cortex by removing all but one whisker, leading to potentiation of the neuronal response to the spared whisker. To determine whether this form of potentiation depends on synaptic plasticity, we studied long-term potentiation (LTP) and sensory-evoked potentials in the barrel cortex of -calcium/calmodulin-dependent protein kinase II (CaMKII)T286A mutant mice. We studied three different forms of LTP induction: theta-burst stimulation, spike pairing, and postsynaptic depolarization paired with low-frequency presynaptic stimulation. None of these protocols produced LTP in CaMKIIT286A mutant mice, although all three were successful in wild-type mice. To study synaptic plasticity in vivo, we measured sensory-evoked potentials in the barrel cortex and found that single-whisker experience selectively potentiated synaptic responses evoked by sensory stimulation of the spared whisker in wild types but not in CaMKIIT286A mice. These results demonstrate that CaMKII autophosphorylation is required for synaptic plasticity in the neocortex, whether induced by a variety of LTP induction paradigms or by manipulation of sensory experience, thereby strengthening the case that the two forms of plasticity are related.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords: barrels ; whiskers ; LTP ; mouse ; synapse ; plasticity
Additional Information: : “Copyright of all material published in The Journal of Neuroscience remains with the authors. The authors grant the Society for Neuroscience an exclusive license to publish their work for the first 6 months. After 6 months the work becomes available to the public to copy, distribute, or display under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license.”http://creativecommons.org/licenses/ See: http://www.jneurosci.org/site/misc/ifa_policies.xhtml
ISSN: 0270-6474
Last Modified: 15 Dec 2017 08:35
URI: http://orca.cf.ac.uk/id/eprint/1143

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