Forbester, Jessica L., Lees, Emily A., Goulding, David, Forrest, Sally, Yeung, Amy, Speak, Anneliese, Clare, Simon, Coomber, Eve L., Mukhopadhyay, Subhankar, Kraiczy, Judith, Schreiber, Fernanda, Lawley, Trevor D., Hancock, Robert E. W., Uhlig, Holm H., Zilbauer, Matthias, Powrie, Fiona and Dougan, Gordon 2018. Interleukin-22 promotes phagolysosomal fusion to induce protection against Salmonella enterica Typhimurium in human epithelial cells. Proceedings of the National Academy of Sciences 115 (40) , pp. 10118-10123. 10.1073/pnas.1811866115 |
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Abstract
Intestinal epithelial cells (IECs) play a key role in regulating immune responses and controlling infection. However, the direct role of IECs in restricting pathogens remains incompletely understood. Here, we provide evidence that IL-22 primed intestinal organoids derived from healthy human induced pluripotent stem cells (hIPSCs) to restrict Salmonella enterica serovar Typhimurium SL1344 infection. A combination of transcriptomics, bacterial invasion assays, and imaging suggests that IL-22–induced antimicrobial activity is driven by increased phagolysosomal fusion in IL-22–pretreated cells. The antimicrobial phenotype was absent in hIPSCs derived from a patient harboring a homozygous mutation in the IL10RB gene that inactivates the IL-22 receptor but was restored by genetically complementing the IL10RB deficiency. This study highlights a mechanism through which the IL-22 pathway facilitates the human intestinal epithelium to control microbial infection.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Medicine |
Publisher: | National Academy of Sciences |
ISSN: | 0027-8424 |
Date of First Compliant Deposit: | 19 September 2018 |
Date of Acceptance: | 8 August 2018 |
Last Modified: | 07 May 2023 08:26 |
URI: | https://orca.cardiff.ac.uk/id/eprint/115097 |
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