Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

TCR deep sequencing of transgenic RAG-1-deficient mice reveals endogenous TCR recombination: a cause for caution

McGuire, Helen M, Watkins, Thomas S, Field, Matthew, Taylor, Sarah, Yasuyama, Nao, Farmer, Andrew, Miles, John J and Fazekas de St. Groth, Barbara 2018. TCR deep sequencing of transgenic RAG-1-deficient mice reveals endogenous TCR recombination: a cause for caution. Immunology and Cell Biology 96 (6) , pp. 642-645. 10.1111/imcb.12033

[img]
Preview
PDF - Accepted Post-Print Version
Download (560kB) | Preview

Abstract

The utility of T‐cell receptor (TCR) transgenic mice in medical research has been considerable, with applications ranging from basic biology all the way to translational and clinical investigations. Crossing of TCR transgenic mice with either recombination‐activating gene (RAG)‐1 or RAG‐2 knockouts is frequently used to generate mice with a monoclonal T‐cell repertoire. However, low level productive TCR rearrangement has been reported in RAG‐deficient mice expressing transgenic TCRs. Using deep sequencing, we set out to directly examine and quantify the presence of these endogenous TCRs. Our demonstration that functional nontransgenic TCRs are present in nonmanipulated mice has wide reaching ramifications worthy of critical consideration.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Nature Publishing Group
ISSN: 0818-9641
Date of First Compliant Deposit: 20 September 2018
Date of Acceptance: 27 February 2018
Last Modified: 22 Sep 2018 07:42
URI: http://orca.cf.ac.uk/id/eprint/115139

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics