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Application of an optimized marker amplification protocol in immunohistochemistry — a valuable tool for visualizing antigenic sites of low signal strength or sparse distribution

von Ruhland, CJ 2018. Application of an optimized marker amplification protocol in immunohistochemistry — a valuable tool for visualizing antigenic sites of low signal strength or sparse distribution. Biotechnic and Histochemistry 93 (7) , pp. 478-484. 10.1080/10520295.2018.1427792

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Abstract

Amplification of immunohistochemical markers received considerable attention during the 1980s and 1990s. The amplification approach was largely abandoned following the development of antigen retrieval and reporter amplification techniques, because the latter were incorporated more easily into high throughput automated procedures in industrial and diagnostic laboratories. There remain, however, a number of instances where marker amplification still has much to offer. Consequently, we examined experimentally the utility of an optimized marker amplification technique in diagnostically relevant tissue where either the original signal strength was low or positive sites were visible, but sparsely distributed. Marker amplification in the former case not only improved the visibility of existing positive sites, but also revealed additional sites that previously were undetectable. In the latter case, positive sites were rendered more intense and therefore more easily seen during low magnification examination of large areas of tissue.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Medicine
Publisher: Informa Healthcare
ISSN: 1052-0295
Date of Acceptance: 10 January 2018
Last Modified: 23 Nov 2018 12:17
URI: http://orca.cf.ac.uk/id/eprint/116521

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