Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Impact of black raspberries on the normal and malignant Apc deficient murine gut microbiome

May, Stephanie, McDermott, Grace, Marchesi, Julian and Parry, Lee 2019. Impact of black raspberries on the normal and malignant Apc deficient murine gut microbiome. Journal of Berry Research 10.3233/JBR-180372

[img]
Preview
PDF - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview

Abstract

BACKGROUND: In animals and humans black raspberries (BRBs) have chemo-preventative effects against Wnt driven colorectal cancer (CRC). While BRBs have made it into clinical trials, the exact mechanisms of BRB action remain unclear. Potentially the chemo-preventative properties are linked to their impact on the gut microbiome, as diet is known to influence the microbial diversity of the gut and plays a key role in regulating intestinal homeostasis and the aetiology of CRC. OBJECTIVE: To determine the impact of a BRB diet on themicrobial biodiversityof thewild-type and malignant mouse intestine. METHODS: Adult mice in which Wnt driven tumourigenesis could be initiated by conditional deletion of Apc in the intestinal stem cell (Lgr5CreERT2) were administered a 10% BRB diet. Total DNA from faecal pellets pre- and post-BRB exposure was used for longitudinal metataxonomic analysis of the V1 to V3 regions of the 16S rRNA gene. RESULTS: Individually BRB intervention and Apc loss alter the microbial community of the gut. In combination, the microbiome changes observed in the Apc deficient intestine are attenuated upon administration of a BRB diet. CONCLUSIONS: Our results suggest that BRB intervention may protectively regulate the gut microbiota in the healthy and malignant intestine.

Item Type: Article
Date Type: Published Online
Status: In Press
Schools: Biosciences
European Cancer Stem Cell Research Institute (ECSCRI)
Publisher: IOS Press
ISSN: 1878-5093
Date of First Compliant Deposit: 3 December 2018
Date of Acceptance: 30 November 2018
Last Modified: 12 Mar 2019 12:36
URI: http://orca.cf.ac.uk/id/eprint/117298

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics