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A Genome-Wide Association Study for Susceptibility to Visual Experience-Induced Myopia

Huang, Y., Kee, C., Hocking, P. M., Williams, C., Guggenheim, Jeremy A. and Yip, S. P. 2019. A Genome-Wide Association Study for Susceptibility to Visual Experience-Induced Myopia. Investigative Ophthalmology & Visual Science 60 (2) , pp. 559-569. 10.1167/iovs.18-25597

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Abstract

Purpose: The rapid rise in prevalence over recent decades and high heritability of myopia suggest a role for gene–environment (G × E) interactions in myopia susceptibility. Few such G × E interactions have been discovered to date. We aimed to test the hypothesis that genetic analysis of susceptibility to visual experience-induced myopia in an animal model would identify novel G × E interaction loci. Methods: Chicks aged 7 days (n = 987) were monocularly deprived of form vision for 4 days. A genome-wide association study (GWAS) was carried out in the 20% of chicks most susceptible and least susceptible to form deprivation (n = 380). There were 304,963 genetic markers tested for association with the degree of induced axial elongation in treated versus control eyes (A-scan ultrasonography). A GWAS candidate region was examined in the following three human cohorts: CREAM consortium (n = 44,192), UK Biobank (n = 95,505), and Avon Longitudinal Study of Parents and Children (ALSPAC; n = 4989). Results: A locus encompassing the genes PIK3CG and PRKAR2B was genome-wide significantly associated with myopia susceptibility in chicks (lead variant rs317386235, P = 9.54e−08). In CREAM and UK Biobank GWAS datasets, PIK3CG and PRKAR2B were enriched for strongly-associated markers (meta-analysis lead variant rs117909394, P = 1.7e−07). In ALSPAC participants, rs117909394 had an age-dependent association with refractive error (−0.22 diopters [D] change over 8 years, P = 5.2e−04) and nearby variant rs17153745 showed evidence of a G × E interaction with time spent reading (effect size −0.23 D, P = 0.022). Conclusions: This work identified the PIK3CG-PRKAR2B locus as a mediator of susceptibility to visually induced myopia in chicks and suggests a role for this locus in conferring susceptibility to myopia in human cohorts.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Publisher: Association for Research in Vision and Ophthalmology (ARVO)
ISSN: 0146-0404
Funders: Hong Kong University Grants Council, NIHR, MRC, Wellcome Trust, University of Bristol, 23andMe
Date of First Compliant Deposit: 5 December 2018
Date of Acceptance: 5 December 2018
Last Modified: 24 Jul 2019 15:21
URI: http://orca.cf.ac.uk/id/eprint/117393

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