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Dissection and preparation of human primary fetal ganglionic eminence tissue for research and clinical applications

Roberton, Victoria, Rosser, Anne E., McGorrian, Anne-Marie and Precious, Sophie V. 2018. Dissection and preparation of human primary fetal ganglionic eminence tissue for research and clinical applications. In: Precious, S., Rosser, A. and Dunnett, S. eds. Huntington’s Disease, Vol. 1780. Methods in Molecular Biology, Humana Press, pp. 573-583. (10.1007/978-1-4939-7825-0_26)

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Abstract

Here, we describe detailed dissection and enzymatic dissociation protocols for the ganglionic eminences from the developing human brain to generate viable quasi-single cell suspensions for subsequent use in transplantation or cell culture. These reliable and reproducible protocols can provide tissue for use in the study of the developing human brain, as well as for the preparation of donor cells for transplantation in Huntington’s disease (HD). For use in the clinic as a therapy for HD, the translation of these protocols from the research laboratory to the GMP suite is described, including modification to reagents used and appropriate monitoring and tissue release criteria.

Item Type: Book Section
Date Type: Published Online
Status: Published
Schools: Medicine
Biosciences
Publisher: Humana Press
ISBN: 9781493978243
ISSN: 1064-3745
Last Modified: 08 Feb 2019 11:33
URI: http://orca.cf.ac.uk/id/eprint/119290

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