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Different forms of African cassava mosaic virus capsid protein within plants and virions

Hipp, Katharina, Zikeli, Kerstin, Kepp, Gabi, Schmid, Lena, Shoeman, Robert L., Jurkowski, Tomasz P., Kleinow, Tatjana and Jeske, Holger 2019. Different forms of African cassava mosaic virus capsid protein within plants and virions. Virology 529 , pp. 81-90. 10.1016/j.virol.2019.01.018

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Abstract

One geminiviral gene encodes the capsid protein (CP), which can appear as several bands after electrophoresis depending on virus and plant. African cassava mosaic virus-Nigeria CP in Nicotiana benthamiana, however, yielded one band (~ 30 kDa) in total protein extracts and purified virions, although its expression in yeast yielded two bands (~ 30, 32 kDa). Mass spectrometry of the complete protein and its tryptic fragments from virions is consistent with a cleaved start M1, acetylated S2, and partial phosphorylation at T12, S25 and S62. Mutants for additional potentially modified sites (N223A; C235A) were fully infectious and formed geminiparticles. Separation in triton acetic acid urea gels confirmed charge changes of the CP between plants and yeast indicating differential phosphorylation. If the CP gene alone was expressed in plants, multiple bands were observed like in yeast. A high turnover rate indicates that post-translational modifications promote CP decay probably via the ubiquitin-triggered proteasomal pathway.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Elsevier
ISSN: 0042-6822
Date of First Compliant Deposit: 18 February 2019
Date of Acceptance: 16 January 2019
Last Modified: 10 Mar 2020 20:06
URI: http://orca.cf.ac.uk/id/eprint/119666

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