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Heritability of ocular component dimensions in mice phenotyped using depth-enhanced swept source optical coherence tomography

Wang, Ling, Povazay, Boris, Chen, Yen Po, Hofer, Bernd, Drexler, Wolfgang and Guggenheim, Jeremy Andrew 2011. Heritability of ocular component dimensions in mice phenotyped using depth-enhanced swept source optical coherence tomography. Experimental Eye Research 93 (4) , pp. 482-490. 10.1016/j.exer.2011.06.008

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Abstract

The range of genetic and genomic resources available makes the mouse a powerful model for the genetic dissection of complex traits. Because accurate, high-throughput phenotypic characterisation is crucial to the success of such endeavours, we recently developed an optical coherence tomography (OCT) system with extended depth range scanning capability for measuring ocular component dimensions in mice. In order to test whether the accuracy and reproducibility of our OCT system was sufficient for gene mapping studies, we carried out an experiment designed to estimate the heritability of mouse ocular component dimensions. High-resolution, two dimensional tomograms were obtained for both eyes of 11 pairs of 8 week-old outbred MF1 mice. Subsequently, images were obtained when their offspring were aged 8 weeks. Biometric data were extracted after image segmentation, reconstruction of the geometric shape of each surface, and calculation of intraocular distances. The repeatability of measurements was evaluated for 12 mice scanned on consecutive days. Heritability estimates were calculated using variance components analysis. Sets of tomograms took ∼2 s to acquire. Biometric data could be obtained for 98% of the 130 eyes scanned. The 95% limits of repeatability ranged from ±6 to ±16 μm for the axial ocular component dimensions. The heritability of the axial ocular components was 0.6–0.8, except for corneal thickness, which had a heritability not significantly different from zero. In conclusion, axial ocular component dimensions are highly heritable in mice, as they are in humans. OCT with extended depth range scanning can be used to rapidly phenotype individual mice with sufficient accuracy and precision to permit gene mapping studies.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Subjects: R Medicine > RE Ophthalmology
Uncontrolled Keywords: OCcular component dimensions ; Animal models ; Optical coherence tomography ; Refractive error ; Myopia; heritability
Publisher: Elsevier
ISSN: 0014-4835
Last Modified: 04 Feb 2018 01:03
URI: http://orca.cf.ac.uk/id/eprint/11985

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