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Bioluminescence of Vibrio fischeri: bacteria respond quickly and sensitively to pulsed microwave electric (but not magnetic) fields

Williams, Catrin F., Geroni, Gilles M., Lloyd, David, Choi, Heungjae, Clark, Nicholas, Pirog, Antoine, Lees, Jonathan and Porch, Adrian 2019. Bioluminescence of Vibrio fischeri: bacteria respond quickly and sensitively to pulsed microwave electric (but not magnetic) fields. Journal of Biomedical Optics 24 (05) , 051412. 10.1117/1.JBO.24.5.051412

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Abstract

Biological systems with intrinsic luminescent properties serve as powerful and noninvasive bioreporters for real-time and label-free monitoring of cell physiology. This study employs the bioluminescent marine bacterium <i>Vibrio fischeri</i> to investigate the effects of separated microwave electric (E) and magnetic (H) fields. Using a cylindrical TM<sub>010</sub> mode aluminum resonant cavity, designed to spatially separate E and H fields of a pulsed microwave (2.45 GHz) input, we sampled at 100-ms intervals the 490-nm emission of bioluminescence from suspensions of the <i>V. fischeri</i>. E-field exposure (at 4.24 and 13.4 kV/m) results in rapid and sensitive responses to 100-ms pulses. H-field excitation elicits no measurable responses, even at 100-fold higher power input levels (equivalent to 183 A/m). The observed effects on bacterial light output partially correlate with measured E-field-induced temperature increases. In conclusion, the endogenous bioluminescence of <i>V. fischeri</i> provides a sensitive and noninvasive method to assess the biological effects of microwave fields.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Engineering
Publisher: Society of Photo-optical Instrumentation Engineers (SPIE)
ISSN: 1083-3668
Date of First Compliant Deposit: 1 March 2019
Date of Acceptance: 21 January 2019
Last Modified: 31 May 2019 20:07
URI: http://orca.cf.ac.uk/id/eprint/120130

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