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Modulation of Candida albicans virulence in in vitro biofilms by oral bacteria

Morse, Daniel, Wilson, Melanie, Wei, Xiao qing, Bradshaw, David, Lewis, Michael and Williams, David 2019. Modulation of Candida albicans virulence in in vitro biofilms by oral bacteria. Letters in Applied Microbiology 68 (4) , pp. 337-343. 10.1111/lam.13145

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Abstract

Candida‐associated denture stomatitis presents as erythema of the palatal mucosa and is caused by biofilms containing the fungus Candida albicans that co‐reside with oral bacteria on the denture‐fitting surface. This study aimed to assess the effect of several frequently encountered oral bacteria on the expression of Candida albicans virulence factors in in vitro polymicrobial biofilms. Biofilms containing C. albicans and selected bacterial species were grown on denture‐acrylic, and analysed by microscopy and by qPCR for expression of putative virulence genes. Candida albicans‐only biofilms showed limited hyphal production. Hyphal development was significantly (P<0·001) increased when biofilms also contained 4 species of oral bacteria (Streptococcus sanguinis, S. gordonii, Actinomyces odontolyticus, and A. viscosus), as was the expression of virulence genes (P<0·05). Importantly, inclusion of Porphyromonas gingivalis in the biofilm consortium resulted in significant (P<0·05) inhibition of virulence gene expression and production of hyphae. In vitro expression of C. albicans virulence factors were modulated in polymicrobial biofilms. The complexity of this modulation is highlighted by the reversal of effects following introduction of a single bacterial species into a biofilm community.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Biosciences
Publisher: Wiley
ISSN: 0266-8254
Date of First Compliant Deposit: 5 March 2019
Date of Acceptance: 26 February 2019
Last Modified: 30 Apr 2019 17:28
URI: http://orca.cf.ac.uk/id/eprint/120227

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