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Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering

Karuna, Arnica, Masia, Francesco, Wiltshire, Marie, Errington, Rachel, Borri, Paola and Langbein, Wolfgang 2019. Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering. Presented at: SPIE BIOS, San Francisco, California, US, 2-7 Feb 2019. Multiphoton Microscopy in the Biomedical Sciences XIX. SPIE, 10.1117/12.2510277

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Abstract

Quantitative determination of the chemical composition of unstained samples, non-invasively, with high three- dimensional spatio-temporal resolution, will accelerate progress in cell biology. The current state of the art in bioimaging is dominated by either chemically non-specific or invasive methods. In this work, we demonstrate label-free, non-invasive quantitative volumetric imaging of human osteosarcoma cells using coherent anti-Stokes Raman scattering microscopy. A data analysis method developed in-house was applied to represent the chemical composition of the cells as volumetric three-dimensional images indicating water, proteins, DNAP (mixture of DNA and proteins), and lipids, and to determine the dry masses of the organic components with picogram resolution.

Item Type: Conference or Workshop Item (Paper)
Date Type: Publication
Status: Published
Schools: Medicine
Biosciences
Physics and Astronomy
Publisher: SPIE
ISSN: Proceedings of SPIE
Date of First Compliant Deposit: 18 March 2019
Last Modified: 31 May 2019 20:19
URI: http://orca.cf.ac.uk/id/eprint/120685

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