Dense genomic sampling identifies highways of pneumococcal recombination

Chewapreecha, Claire, Harris, Simon R, Croucher, Nicholas J, Turner, Claudia, Marttinen, Pekka, Cheng, Lu ORCID: https://orcid.org/0000-0002-6391-2360, Pessia, Alberto, Aanensen, David M, Mather, Alison E, Page, Andrew J, Salter, Susannah J, Harris, David, Nosten, Francois, Goldblatt, David, Corander, Jukka, Parkhill, Julian, Turner, Paul and Bentley, Stephen D 2014. Dense genomic sampling identifies highways of pneumococcal recombination. Nature Genetics 46 (3) , pp. 305-311. 10.1038/ng.2895

Full text not available from this repository.

Abstract

Evasion of clinical interventions by Streptococcus pneumoniae occurs through selection of non-susceptible genomic variants. We report whole-genome sequencing of 3,085 pneumococcal carriage isolates from a 2.4-km2 refugee camp. This sequencing provides unprecedented resolution of the process of recombination and its impact on population evolution. Genomic recombination hotspots show remarkable consistency between lineages, indicating common selective pressures acting at certain loci, particularly those associated with antibiotic resistance. Temporal changes in antibiotic consumption are reflected in changes in recombination trends, demonstrating rapid spread of resistance when selective pressure is high. The highest frequencies of receipt and donation of recombined DNA fragments were observed in non-encapsulated lineages, implying that this largely overlooked pneumococcal group, which is beyond the reach of current vaccines, may have a major role in genetic exchange and the adaptation of the species as a whole. These findings advance understanding of pneumococcal population dynamics and provide information for the design of future intervention strategies.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Nature Publishing Group
ISSN:	1061-4036
Date of Acceptance:	15 January 2014
Last Modified:	25 Oct 2022 13:46
URI:	https://orca.cardiff.ac.uk/id/eprint/120716

Citation Data

Cited 253 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item