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In vitro neuroprotective activities of two distinct probiotic consortia

Michael, D.R., Davies, T.S., Loxley, K.E., Allen, M.D., Good, M.A., Hughes, T.R. and Plummer, S.F. 2019. In vitro neuroprotective activities of two distinct probiotic consortia. Beneficial Microbes 10 (4) , pp. 437-447. 10.3920/BM2018.0105

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Abstract

Neurodegeneration has been linked to changes in the gut microbiota and this study compares the neuroprotective capability of two bacterial consortia, known as Lab4 and Lab4b, using the established SH-SY5Y neuronal cell model. Firstly, varying total antioxidant capacities (TAC) were identified in the intact cells from each consortia and their secreted metabolites, referred to as conditioned media (CM). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Crystal Violet (CV) assays of cell viability revealed that Lab4 CM and Lab4b CM could induce similar levels of proliferation in SH-SY5Y cells and, despite divergent TAC, possessed a comparable ability to protect undifferentiated and retinoic acid-differentiated cells from the cytotoxic actions of rotenone and undifferentiated cells from the cytotoxic actions of 1-methyl-4-phenylpyridinium iodide (MPP+). Lab4 CM and Lab4b CM also had the ability to attenuate rotenone-induced apoptosis and necrosis with Lab4b inducing the greater effect. Both consortia showed an analogous ability to attenuate intracellular reactive oxygen species accumulation in SH-SY5Y cells although the differential upregulation of genes encoding glutathione reductase and superoxide dismutase by Lab4 CM and Lab4b CM, respectively, implicates the involvement of consortia-specific antioxidative mechanisms of action. This study implicates Lab4 and Lab4b as potential neuroprotective agents and justifies their inclusion in further in vivo studies.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Psychology
Medicine
Publisher: Wageningen Academic Publishers
ISSN: 1876-2883
Date of First Compliant Deposit: 19 March 2019
Date of Acceptance: 11 December 2018
Last Modified: 05 Jun 2019 14:16
URI: http://orca.cf.ac.uk/id/eprint/120871

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