Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Crystal structure and biophysical analysis of furfural detoxifying aldehyde reductase from clostridium beijerinkii

Scott, Alan F., Cresser-Brown, Joel, Williams, Thomas L., Rizkallah, Pierre J., Jin, Yi, Luk, Louis Y.-P. and Allemann, Rudolf K. 2019. Crystal structure and biophysical analysis of furfural detoxifying aldehyde reductase from clostridium beijerinkii. Applied and Environmental Microbiology , -. 10.1128/AEM.00978-19
Item availability restricted.

[img] PDF - Accepted Post-Print Version
Restricted to Repository staff only until 17 November 2019 due to copyright restrictions.

Download (3MB)

Abstract

Many aldehydes such as furfural are present in high quantities in lignocellulose lysates and are fermentation inhibitors that make biofuel production from this abundant carbon source extremely challenging. Cbei_3974 has recently been identified as an aldo-keto reductase responsible for partial furfural resistance in Clostridium beijerinkii. Rational engineering of this enzyme could enhance the furfural tolerance of this organism thereby improving biofuel yields. We report an extensive characterization of Cbei_3974 and a single crystal X-ray structure of Cbei_3974 in complex with NADPH at a resolution of 1.75 Å. Docking studies identified residues involved in substrate binding and an activity screen revealed the substrate tolerance of the enzyme. Hydride transfer, which is partially rate limiting under physiological conditions, occurs from the pro-R hydrogen of NADPH. Enzyme isotope labeling revealed a temperature-independent enzyme isotope effect of unity, indicating that the enzyme does not use dynamic coupling for catalysis and suggests that the active site of the enzyme is optimally configured for catalysis with the substrate tested.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Chemistry
Publisher: American Society for Microbiology
ISSN: 0099-2240
Funders: BBSRC
Date of First Compliant Deposit: 23 May 2019
Date of Acceptance: 8 May 2019
Last Modified: 27 Jun 2019 20:34
URI: http://orca.cf.ac.uk/id/eprint/122822

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics