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Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration

Munir, Arooj, Døskeland, Anne, Avery, Steven J., Fuoco, Tiziana, Mohamed-Ahmed, Samih, Lygre, Henning, Finne-Wistrand, Anna, Sloan, Alastair J., Waddington, Rachel J., Mustafa, Kamal and Suliman, Salwa 2019. Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration. Journal of Tissue Engineering 10 , p. 204173141985270. 10.1177/2041731419852703

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Abstract

Poly(L-lactide-co-ε-caprolactone) scaffolds were functionalised by 10 or 20 µg/mL of human demineralised dentine matrix. Release kinetics up to 21 days and their osteogenic potential on human bone marrow stromal cells after 7 and 21 days were studied. A total of 390 proteins were identified by mass spectrometry. Bone regeneration proteins showed initial burst of release. Human bone marrow stromal cells were cultured on scaffolds physisorbed with 20 µg/mL and cultured in basal medium (DDM group) or physisorbed and cultured in osteogenic medium or cultured on non-functionalised scaffolds in osteogenic medium. The human bone marrow stromal cells proliferated less in demineralised dentine matrix group and activated ERK/1/2 after both time points. Cells on DDM group showed highest expression of IL-6 and IL-8 at 7 days and expressed higher collagen type 1 alpha 2, SPP1 and bone morphogenetic protein-2 until 21 days. Extracellular protein revealed higher collagen type 1 and bone morphogenetic protein-2 at 21 days in demineralised dentine matrix group. Cells on DDM group showed signs of mineralisation. The functionalised scaffolds were able to stimulate osteogenic differentiation of human bone marrow stromal cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Publisher: SAGE Publications
ISSN: 2041-7314
Date of First Compliant Deposit: 3 June 2019
Date of Acceptance: 3 May 2019
Last Modified: 03 Jun 2019 12:48
URI: http://orca.cf.ac.uk/id/eprint/123086

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