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The Ussing chamber system for measuring intestinal permeability in health and disease

Thomson, Amanda, Smart, Kathryn, Somerville, Michelle S., Lauder, Sarah N., Appanna, Gautham, Horwood, James, Lawrence-Sunder, Raj, Srivastava, Brijesh, Durai, Dhamaraj, Scurr, Martin J., Keita, Asa V., Gallimore, Awen M. and Godkin, Andrew 2019. The Ussing chamber system for measuring intestinal permeability in health and disease. BMC Gastroenterology 19 , 98. 10.1186/s12876-019-1002-4

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Abstract

Background The relationship between intestinal epithelial integrity and the development of intestinal disease is of increasing interest. A reduction in mucosal integrity has been associated with ulcerative colitis, Crohn’s disease and potentially could have links with colorectal cancer development. The Ussing chamber system can be utilised as a valuable tool for measuring gut integrity. Here we describe step-by-step methodology required to measure intestinal permeability of both mouse and human colonic tissue samples ex vivo, using the latest equipment and software. This system can be modified to accommodate other tissues. Methods An Ussing chamber was constructed and adapted to support both mouse and human tissue to measure intestinal permeability, using paracellular flux and electrical measurements. Two mouse models of intestinal inflammation (dextran sodium sulphate treatment and T regulatory cell depletion using C57BL/6-FoxP3DTR mice) were used to validate the system along with human colonic biopsy samples. Results Distinct regional differences in permeability were consistently identified within mouse and healthy human colon. In particular, mice showed increased permeability in the mid colonic region. In humans the left colon is more permeable than the right. Furthermore, inflammatory conditions induced chemically or due to autoimmunity reduced intestinal integrity, validating the use of the system. Conclusions The Ussing chamber has been used for many years to measure barrier function. However, a clear and informative methods paper describing the setup of modern equipment and step-by-step procedure to measure mouse and human intestinal permeability isn’t available. The Ussing chamber system methodology we describe provides such detail to guide investigation of gut integrity.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Additional Information: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Publisher: BioMed Central
ISSN: 1471-230X
Funders: Wellcome Trust
Date of First Compliant Deposit: 18 June 2019
Date of Acceptance: 28 May 2019
Last Modified: 28 Jul 2020 01:29
URI: http://orca.cf.ac.uk/id/eprint/123497

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