Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Long wavelength TCF-based fluorescent probe for the detection of alkaline phosphatase in live cells

Gwynne, Lauren, Sedgwick, Adam C., Gardiner, Jordan E., Williams, George T., Kim, Gyoungmi, Lowe, John P., Maillard, Jean-Yves, Jenkins, A. Toby A., Bull, Steven D., Sessler, Jonathan L., Yoon, Juyoung and James, Tony D. 2019. Long wavelength TCF-based fluorescent probe for the detection of alkaline phosphatase in live cells. Frontiers in Chemistry 7 , -. 10.3389/fchem.2019.00255

[img]
Preview
PDF - Accepted Post-Print Version
Download (1MB) | Preview

Abstract

A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence “turn on” (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Pharmacy
Publisher: Frontiers Media
ISSN: 2296-2646
Date of First Compliant Deposit: 24 June 2019
Date of Acceptance: 1 April 2019
Last Modified: 19 Oct 2019 05:20
URI: http://orca.cf.ac.uk/id/eprint/123682

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics