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Molecular community profiling of the bacterial microbiota associated with denture-related stomatitis

Morse, Daniel, Smith, Ann, Wilson, Melanie, Marsh, Lucy, White, Lewis, Posso, Raquel, Bradshaw, David, Wei, Xiao Qing, Lewis, Michael and Williams, David 2019. Molecular community profiling of the bacterial microbiota associated with denture-related stomatitis. Scientific Reports 9 , 10228. 10.1038/s41598-019-46494-0

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Abstract

Denture-associated stomatitis (DS) affects over two-thirds of denture-wearers. DS presents as erythema of the palatal mucosa in areas where denture-surface associated polymicrobial biofilms containing the fungus Candida albicans exist. The contribution of the oral bacterial microbiota toward the infection is unknown. Therefore, this study characterised the bacterial microbiota of sites within the oral cavity to identify potential associations with occurrence of DS. Denture-wearing patients were recruited (denture stomatitis (DS) n = 8; non-denture stomatitis (NoDS) n = 11) and the oral bacterial microbiota of the tongue, palate and denture-fitting surface was characterised using next-generation sequencing. Operational taxonomic units (OTUs) were identified to bacterial genera and species, and presence/absence and relative abundances were examined. A significant (P = 0.007) decrease in the number of OTUs and thus, diversity of the microbiota was observed in tongue samples of DS patients (vs non-DS). The microbiota of denture-fitting surfaces and palatal mucosae were similar. Large differences in the abundance of bacterial genera and species were observed at each sample site, and unique presence/absence of bacteria was noted. Presence/absence and relative abundance of specific bacteria associated with DS warrants further in vitro and in vivo evaluation, particularly as our previous work has shown C. albicans virulence factor modulation by oral bacteria.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Biosciences
Medicine
Publisher: Nature Publishing Group
ISSN: 2045-2322
Funders: EPSRC
Date of First Compliant Deposit: 28 June 2019
Date of Acceptance: 27 June 2019
Last Modified: 16 Aug 2019 13:50
URI: http://orca.cf.ac.uk/id/eprint/123800

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