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A compact microwave applicator for the rapid detection of clostridium difficile

Hamzah, H., Ahortor, E., Malyshev, D., Choi, H., Lees, J., Baillie, L. and Porch, A. 2019. A compact microwave applicator for the rapid detection of clostridium difficile. Presented at: 2019 IEEE MTT-S International Microwave Biomedical Conference (IMBioC), Nanjing, China, 6-8 May 2019. 2019 IEEE MTT-S International Microwave Biomedical Conference (IMBioC). IEEE, 10.1109/IMBIOC.2019.8777882

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Abstract

Clostridium difficile is a major cause of hospital acquired infection that poses significant diagnostic challenges. In this paper, a new type of resonant microwave applicator is proposed for the liberation of DNA from C. difficile spores via microwave disruption, followed by rapid detection using a sandwich hybridization assay. A split-ring resonator is designed to operate at 2.4 GHz with a 3 mm active gap region that contains an isolate volume of approximately 10 mm 3 , exposed to pulsed microwaves of 12 W rms power. The parallel electric field configuration maximizes the interaction between the microwaves and the sample. In a proof of principle study, in combination with pathogen specific DNA probes, we have used the system to correctly identify virulent strains of C. difficile using magnetic bead extraction of DNA suitable for point-of-care application

Item Type: Conference or Workshop Item (Paper)
Date Type: Published Online
Status: Published
Schools: Pharmacy
Engineering
Publisher: IEEE
ISBN: 978-1-5386-7395-9
Date of First Compliant Deposit: 28 August 2019
Date of Acceptance: 26 January 2019
Last Modified: 30 Nov 2019 06:42
URI: http://orca.cf.ac.uk/id/eprint/125152

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